1. Super-Resolution Fluorescence Microscopy
The nanoscale resolution of super-resolution microscopy is especially suitable for nondestructively detecting dynamic process in nanostructures. Among super-resolution microscopy techniques, (direct) stochastic optical reconstruction microscopy ((d)STORM), which is based on detection and localization of the signal of a single fluorescent molecule, can achieve 20–30 nm lateral resolution. This technique has been widely used, for example, in detecting the spatial distribution of organic and inorganic phases, in distinguishing the nanoscale structures in cell compartments like the nucleus and cell organelles, or acquiring the distributions of proteins on cell membranes. Angew. Chem. Int. Ed., 2016, 55, 908-911; Sci. Rep., 2015, 5, 16559; Sci. Rep., 2015, 5, 11073.
Direct Observation of the Distribution of Gelatin in CaCO3 Crystals
Detailed Structures of Tubular Compartments in NIH3T3 Cells